Wenqi Li Ph.D
Dr. Li’s research is mainly focused on the study of proteins preparation and characterization. He is proficient in the technology of preparation and characterization of biological macromolecules. He has professional experiences in the equipment development of life science. He has successfully invented an automatic platform for protein separation and purification. He is committed to the development of instruments and domestic substitution. The density gradient automaker for ultracentrifugation has been in progress to about half and is planned to be completed in the next year. Dr. Li is well known for his pioneering work as one of the national leaders in the area of analytical ultracentrifugation (AUC): He has led the first and second China AUC Conference, organized the publishment of the first AUC book in Chinese, and published a number of papers on AUC related techniques and methods. He is organizing the development of AUC data analysis and processing software, and leading the developm
Resume
2000-2004Department of Life Science and Technology, Beijing University of Chemical Technology B.S.
2004-2007Institute of Biophysics, Chinese Academy of Sciences Master
2007-2011School of Life Sciences, Tsinghua University Lab Manager
2011-2014School of Life Sciences, Tsinghua University Ph.D
2014-2016School of Medicine, Tsinghua University Postdoc
2019-2023National Protein Science Facility (Tsinghua, Beijing ) Associate Director
2013-PresentSchool of Life Sciences, Tsinghua University Core facility Director
Main Research Fields
The preparation, purification and identification of protein is the basis of all protein scientific research. the Core Facility for Biomolecule Preparation and Characterization at Technology Center for Protein Science, Tsinghua University have the scale effect since foundation, which can give full support to the research on protein structure and function. We provide four kinds of expression systems such as: E. Coli, Yeast, Insect cell and Mammalian cell suspended system to researchers from universities, research institutes, small biological companies and so on. We can also provide the protein related services, including molecular cloning, expression and purification, characterization，analysis of interaction，Screening of small molecule compounds and a series of related research.
Currently we have a large number of expression vectors containing different tags (His, GST, etc.), effective expression strain and cell lines. We also provide a variety of specific bioreactors (5 l - 100 l) in order to support the user for high quality samples and other specific expression requirements. We also have more than ten types of AKTA instruments and different kinds of GE columns for protein purification, which can provide large-scale protein purification at the same time. To study the protein physical/chemical properties and interactions between the bio-molecules, the facility also equipped with more than ten kinds of large instruments, such as: AUC, DLS, SLS, UV, CD, Stop-flow, ITC, BLI, SPRi, MST and so on.
The Core Facility for Biomolecule Preparation and Characterization has has 12 staff members who are responsible for the daily use, management and testing services of the instruments. For more information and fee details, please login to the facility website：http://phoenix.tsinghua.edu.cn/ptxx/swypzbyjdpt.htm
Selected Publications
1. Linjie Yuan, Xianqiang Ma, Yunyun Yang, et.al., Wenqi Li, Yonghui Zhang. Phosphoantigens glue butyrophilin 3A1 and 2A1 to activate Vγ9Vδ2 T cells. Nature. (2023) 621(7980):840-848.
2. Yafei Li, ShenYang Wu, Wendan Chu, Cuiyan Zhou and Wenqi Li*. Poly Acidic Amino Acids Sequence Drives Variation of Protein in Hydrodynamic Properties. Journal of Biology, (2023) 40 (1): 21-26 (*, Corresponding Author)
3. Chang Qing, Yu Minda, Li Wenqi*. Recombinant expression and purification of the extracellular domain of human B lymphocyte surface antigen CD20. Journal of Biology, (2022) 39(06) : 20-24 (*, Corresponding Author)
4. Wendan Chu, Yafei Lu and Wenqi Li*. Analysis of protein Self-association by sedimentation velocity. Experimental Technology and Management. (2022)39(5):9-14. (*, Corresponding Author)
5. Cuiyan Zhou, Minda Yu, Wenqi Li*. Comparation of the Methods for Measuring Protein’s Thermal Unfolding. Analysis and Testing Technology and Instruments. (2021). 27(4):252-259. (*, Corresponding Author)
6. Dongdong Li, Wendan Chu, Xinlei Sheng*, and Wenqi Li*. Optimization of membrane protein TmrA purification procedure guided by Analytical Ultracentrifugation. Membranes (Basel). (2021). 11(10):780. (*, Corresponding Author)
7. Chang, Q. and Li, W. Q*. High Throughput Screening of the Epigenetic Interactome by Surface Plasmon Resonance Imaging (SPRi). Bio-101: e1010842. DOI: 10.21769/BioProtoc.1010842. (2021). (*, Corresponding Author)
8. Chang, Q., Yang, Z., and Li, W. Q*. High-Throughput Screening of Small-molecular Targeting Biomolecular by Biacore8K+. Bio-101: e1010846. DOI: 10.21769/BioProtoc.1010846. (2021) (*, Corresponding Author)
9. Dongdong Li, Wendan Chu, Wenqi Li*. Application of analytical ultracentrifugation technology in protein research[J]. Experimental Technology and Management. (2021) 38(7): 36-43. (*, Corresponding Author)
10. Huaying Zhao, Wenqi Li, Wendan Chu, Mary Bollard, Regina Adão, Peter Schuck. Quantitative Analysis of Protein Self-Association by Sedimentation Velocity [J]. Curr Protoc Protein Sci. (2020 ) 101(1): e109.
11. Dan Tang, Jingwen Sheng, Liangting Xu, Xiechao Zhan, Jiaming Liu, Hui Jiang, Xiaoling Shu, Xiaoyu Liu, Tizhong Zhang, Lan Jiang, Cuiyan Zhou, Wenqi Li, Wei Cheng, Zhonghan Li, Kunjie Wang, Kefeng Lu, Chuangye Yan, Shiqian Qi. Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP for Rab8a and Rab11a. PNAS. (2020) 117 (18) :9876-9883
12. Dan Tang, Chunjing Chen, Ga Liao, Jiaming Liu, Banghua Liao, QingQing Huang, Qianqian Chen, Jiahui Zhao, Hui Jiang, Jinsong Duan, Jin Huang, Kunjie Wang, Jiawei Wang, Cuiyan Zhou, Wendan Chu, Wenqi Li, Bo Sun, Zhonghan Li, Lunzhi Dai, Xianghui Fu, Wei Cheng, Yuhua Xue, Shiqian Qi. Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription[J]. Cell Discovery. (2020)6:7.
13. CHU Wen-Dan, XU Yang, ZHOU Cui-Yan, LU Ya-Fei, YU Xiao-Xia, ZHANG Rui-Xuan, LI Wen-Qi. Study of Membrane Protein TmrAB by Analytical Ultracentrifugation. Progress in Biochemistry and Biophysics. (2018)45(10): 1047-1053. (*, Corresponding Author)
14. Lu Chen， Qianqian Feng， Jiao Jin，Xun Pan，Yongheng Jiang ，Li Wenqi *. Analysis and sugestions on curent bio-waste disposal in some universities. Experimental Technology and Management. (2017)34(11):268-271. (*, Corresponding Author)
15. Xin Gong, Hongwu Qian, Wei Shao, Jingxian Li, Jianping Wu, Wenqi Li, Peter Espenshade, Liu Jun-Jie, Wang Hong-Wei and Nieng Yan. Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization. Cell Research. (2016) 26(11):1197-1211. (Impact factor: 10.526)
16. CHU WenDan, ZHOU CuiYan, LU YaFei, XU Yang, LI WenQi*. Analytical ultracentrifugation reveals poly acidic amino acids sequence alters hydrodynamic properties of SnRK2.6. Progress in Biochemistry and Biophysics. (2016) 43(3):256-264. (*,Corresponding Author)
17. Zhongchao Gai, Wendan Chu, Wei Deng, Wenqi Li, Hua Li, Ailiang He, Mark Nellist, and Geng Wu. Structure of the TBC1D7−TSC1 complex reveals that TBC1D7 stabilizes dimerization of the TSC1 C-terminal coiled coil region, Journal of Molecular Cell Biology. (2016) 8(5):411-425. (Impact factor: 5.98).
18. Wenqi Li*, Li Wang*, Xinlei Sheng*, Chuangye Yan, Rui Zhou, Jing Hang, Ping Yin, Nieng Yan. Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13. Cell Research. (2013) 23:1369-1379. (*, Co-first author). (Impact factor: 10.526).
19. Wenqi Li*, Yexing Liu*, Xinlei Sheng*, Ping Yin, Feizhuo Hu, Ying Liu, Chen Chen, Quanxiu Li, Chuangye Yan, Jiawei Wang. Structure and mechanism of a type III secretion protease, NleC. Acta Crystallogr D Biol Crystallogr. (2014) D70:40-47. (*, Co-first author). (Impact factor: 14.103).
20. Qi Hao*, Ping Yin*, Wenqi Li*, Li Wang, Chuangye Yan, Zhaohu Lin, Jim Zhen Wu, Jiawei Wang, S. Frank Yan, and Nieng Yan . The molecular basis of ABA-independent inhibition of PP2Cs by a subclass of PYL proteins. Mol Cell. (2011)42(5):662-72. (*, Co-first author). (Impact factor: 15.28).
21. Quanxiu Li, Chuangye Yan, Huisha Xu, Zheng Wang, Jiafu Long, Wenqi Li , Jianping Wu, Ping Yin, Nieng Yan, Examination of the Dimerization States of the Single-stranded RNA-recognition Protein PPR10, J Biol Chem. (2014)289(45).
22. Yuan He, Qi Hao, Wenqi Li, Chuangye Yan, Ning Yan, Ping Yin. Identification and Characterization of ABA Receptors in Oryza sativa. PLoS One. (2014) 9(4).
23. Tian Xie, Ruobing Ren, Yuan-yuan Zhang, Yuxuan Pang, Chuangye Yan, Xinqi Gong, Yuan He, Wenqi Li, Di Miao, Qi Hao, Haiteng Deng, Zhixin Wang, Jia-wei Wu, and Nieng Yan. Molecular mechanism for inhibition of a critical component in the Arabidopsis thaliana abscisic acid signal transduction pathways, SnRK2.6, by protein phosphatase ABI1. J Biol Chem. (2012) 287(1):794-802. (Impact factor: 4.651).
24. Peilong Lu, Guifeng Lu, Chuangye Yan, Li Wang, Wenqi Li, and Ping Yin. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. (2012 )441 (2):591-7. (Impact factor: 4.654).
25. Qi Hao, Ping Yin, Chuangye Yan, Xiaoqiu Yuan, Wenqi Li, Zhiping Zhang, Lei Liu, Jiawei Wang, and Nieng Yan. Functional mechanism of the abscisic acid agonist pyrabactin. J Biol Chem. (2010 )285(37):28946-52. (Impact factor: 4.651).
26. Ping Yin, He Fan, Qi Hao, Xiaoqiu Yuan, Di Wu, Yuxuan Pang, Chuangye Yan, Wenqi Li Jiawei Wang, and Nieng Yan. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nat Struct Mol Biol. (2009)16(12):1230-6. (Impact factor: 11.902).
