联系方式

电话:+86-10-62782031

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邮箱:liwenqi@tsinghua.edu.cn

李文奇博士

蛋白质制备与鉴定平台主管

个人履历

2000-2004北京化工大学生命科学学院 学士

2004-2007中国科学院生物物理研究所 硕士

2007-2011清华大学生命科学学院 实验室管理员

2011-2014清华大学生命科学学院 博士

2014-2016清华大学医学院 博士后

2019-2023国家蛋白质科学研究(北京)设施清华基地 副主任

2013-至今清华大学生命科学学院 平台主管

主要科研领域与方向

蛋白质的表达纯化与理化性质分析。熟悉原核、酵母、昆虫细胞、哺乳动物细胞等蛋白表达系统,以及蛋白质无标签纯化、亲和标签纯化、活性组分纯化等多种分离纯化手段;熟练掌握发酵工程工艺;精通紫外(UV)、荧光(FL)、圆二色光谱(CD)、动态光散射(DLS)、静态光散射(sls)、停留技术(Stop-flow)、差示扫描量热技术(DSC)、等温滴定量热技术(ITC)、生物膜干涉技术(BLI)、表面等离子共振技术(SPR)、分析超速离心技术(AUC)、微量热泳动技术(MST)等多种理化性质分析技术。

代表性论文

1. 褚文丹,芦亚菲,李文奇*. 沉降速率法研究蛋白质自聚集. 实验技术与管理. (2022) 39(5):9-14. (*, 通讯作者)
2. 周翠燕,俞敏达,李文奇*. 三种测定蛋白质热稳定性方法的比较. 分析测试技术与仪器. (2021) 27(4):252-259. (*, 通讯作者)
3. 李亚妃,武沈阳,褚文丹,周翠燕,施清华,李文奇*.多聚酸性氨基酸对蛋白质性质的影响. 生物学杂志. (2021)已录用 (*, 通讯作者)
4. Dongdong Li, Wendan Chu, Xinlei Sheng *, and Wenqi Li *. Optimization of membrane protein TmrA purification procedure guided by Analytical Ultracentrifugation. Membranes (Basel). (2021)11(10):780. (*, Corresponding Author)
5. 常卿,俞敏达,李文奇*.一种人B淋巴细胞表面抗原的重组表达和纯化. 生物学杂志. (2021)已录用(*, 通讯作者)
6. 常卿,李文奇*. 利用表面等离子共振成像技术高通量筛选表观遗传相互作用. Bio-101 e1010842. Doi: 10.21769/BioProtoc. 1010842. (2021) .(*, 通讯作者)
7. 常卿,杨姊,李文奇*. 利用Biacore8K+高通量筛选靶向生物大分子的小分子活性物质. Bio-101: e1010846. DOI: 10.21769/BioProtoc.1010846. (2021). (*, 通讯作者)
8. 李冬冬,褚文丹,李文奇*. 分析超速离心技术在蛋白质研究中的应用. 实验技术与管理. (2021)38(7): 36-43(*, 通讯作者)
9. Huaying Zhao, Wenqi Li , Wendan Chu, Mary Bollard, Regina Adão, Peter Schuck.  Quantitative Analysis of Protein Self-Association by Sedimentation Velocity [J]. Curr Protoc Protein Sci. (2020)101(1): e109.
10. Dan Tang, Jingwen Sheng, Liangting Xu, Xiechao Zhan, Jiaming Liu, Hui Jiang, Xiaoling Shu, Xiaoyu Liu, Tizhong Zhang, Lan Jiang, Cuiyan Zhou, Wenqi Li *, Wei Cheng, Zhonghan Li, Kunjie Wang, Kefeng Lu, Chuangye Yan, Shiqian Qi. Cryo-EM structure of C9ORF72-SMCR8-WDR41 reveals the role as a GAP for Rab8a and Rab11a. PNAS. (2020) 117 (18) :9876-9883
11. Dan Tang, Chunjing Chen, Ga Liao, Jiaming Liu, Banghua Liao, QingQing Huang, Qianqian Chen, Jiahui Zhao, Hui Jiang, Jinsong Duan, Jin Huang, Kunjie Wang, Jiawei Wang, Cuiyan Zhou, Wendan Chu, Wenqi Li , Bo Sun, Zhonghan Li, Lunzhi Dai, Xianghui Fu, Wei Cheng, Yuhua Xue, Shiqian Qi. Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription[J]. Cell Discovery, (2020)6:7.
12. 褚文丹,徐扬,周翠燕,芦亚菲,于晓霞,张瑞轩,李文奇*. 分析超速离心技术研究膜蛋白TmrAB. 生物化学与生物物理进展. (2018) 45(10): 1047-1053. (*, 通讯作者)
13. 陈璐,冯倩倩,靳娇,潘勋,江永亨,李文奇*. 部分高校生物废弃物处置现状分析与建议. 实验技术与管理. (2017)34(11):268-271. (*, 通讯作者)
14. Xin Gong, Hongwu Qian, Wei Shao, Jingxian Li, Jianping Wu, Wenqi Li , Peter Espenshade, Liu Jun-Jie, Wang Hong-Wei and Nieng Yan. Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization. Cell Research. (2016) 26(11):1197-1211.
15. 褚文丹,周翠燕,芦亚菲,徐扬,李文奇*. 分析超速离心研究多聚酸性氨基酸对SnRK2.6 的影响. 生物化学与生物物理进展. (2016)43(3):256-264. (*, 通讯作者)
16. Zhongchao Gai, Wendan Chu, Wei Deng, Wenqi Li, Hua Li, Ailiang He, Mark Nellist, and Geng Wu. Structure of the TBC1D7−TSC1 complex reveals that TBC1D7 stabilizes dimerization of the TSC1 C-terminal coiled coil region, Journal of Molecular Cell Biology. (2016) 8(5):411-425.
17. Wenqi Li*, Li Wang*, Xinlei Sheng*, Chuangye Yan, Rui Zhou, Jing Hang, Ping Yin, Nieng Yan. Molecular basis for the selective and ABA-independent inhibition of PP2CA by PYL13. Cell Research. (2013) 23:1369-1379. (*, Co-first author). 
18. Wenqi Li*, Yexing Liu*, Xinlei Sheng*, Ping Yin, Feizhuo Hu, Ying Liu, Chen Chen, Quanxiu Li, Chuangye Yan, Jiawei Wang. Structure and mechanism of a type III secretion protease, NleC. Acta Crystallogr D Biol Crystallogr. (2014) D70:40-47. (*, Co-first author).
19. Qi Hao*, Ping Yin*, Wenqi Li*, Li Wang, Chuangye Yan, Zhaohu Lin, Jim Zhen Wu, Jiawei Wang, S. Frank Yan, and Nieng Yan . The molecular basis of ABA-independent inhibition of PP2Cs by a subclass of PYL proteins. Mol Cell. (2011)42(5):662-72. (*, Co-first author).
20. Quanxiu Li, Chuangye Yan, Huisha Xu, Zheng Wang, Jiafu Long, Wenqi Li , Jianping Wu, Ping Yin, Nieng Yan, Examination of the Dimerization States of the Single-stranded RNA-recognition Protein PPR10, J Biol Chem. (2014) 289(45).
21. Yuan He, Qi Hao, Wenqi Li, Chuangye Yan, Ning Yan, Ping Yin. Identification and Characterization of ABA Receptors in Oryza sativa. PLoS One. (2014 ) 9(4). 
22. Tian Xie, Ruobing Ren, Yuan-yuan Zhang, Yuxuan Pang, Chuangye Yan, Xinqi Gong, Yuan He, Wenqi Li, Di Miao, Qi Hao, Haiteng Deng, Zhixin Wang, Jia-wei Wu, and Nieng Yan. Molecular mechanism for inhibition of a critical component in the Arabidopsis thaliana abscisic acid signal transduction pathways, SnRK2.6, by protein phosphatase ABI1. J Biol Chem. (2012)287(1):794-802. 
23. Peilong Lu, Guifeng Lu, Chuangye Yan, Li Wang, Wenqi Li, and Ping Yin. Structure of the mRNA splicing complex component Cwc2: insights into RNA recognition. Biochem J. (2012) 441 (2):591-7.
24. Qi Hao, Ping Yin, Chuangye Yan, Xiaoqiu Yuan, Wenqi Li, Zhiping Zhang, Lei Liu, Jiawei Wang, and Nieng Yan. Functional mechanism of the abscisic acid agonist pyrabactin. J Biol Chem. (2010) 285(37):28946-52.
25. Ping Yin, He Fan, Qi Hao, Xiaoqiu Yuan, Di Wu, Yuxuan Pang, Chuangye Yan, Wenqi Li Jiawei Wang, and Nieng Yan. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nat Struct Mol Biol. (2009)(12):1230-6.