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电话:62784919

邮箱:liunian88@tsinghua.edu.cn

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刘念博士

2020 拜耳研究员
2016 Jane Coffin Childs医学研究纪念基金
2016 国际RNA学会Scaringe奖
2016 自费留学生奖学金
2015 Aldrich Alfred R. Bader 创新奖
2010 郭沫若奖

个人履历

2006-2010 中国科学技术大学高分子化学 学士

2010-2015 芝加哥大学化学系 博士

2015-2019 斯坦福大学系统生物学系 博士后研究员

2019-至今 清华大学生命科学学院 特聘助理教授

主要科研领域与方向

转座子是可以移动(或转座)的DNA,存在于几乎所有的生命体中,属于基因组中的“暗物质”。转座子在人体内大量富集,占据大约一半的人体DNA,并且仍然具有转座活性。人体转座子的活性与胚胎发育、神经发育以及上百种疾病密切相关,但是其相关机理和功能至今不是非常清楚。我们利用分子、细胞、生化、高通量测序和生物统计等手段主攻转座子活性是如何被调控的机理,及其在发育和疾病中的具体功能。

代表性论文

1. Zuo F#, Jiang J#, Fu H#, Yan K, Liefke R, Zhang J, Hong Y, Chang Z, Liu N, Wang Z*, Xi Q*. A TRIM66/DAX1/Dux axis suppresses the totipotent 2-cell-like state in murine embryonic stem cells. Cell Stem Cell. 29(6), 948-961 (2022).

2. Liu N#, Lee C#, Swigut T, Grow E, Gu B, Bassik M, Wysocka J. Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulators. Nature 553, 228-232 (2018).

3. Liu N#, Zhou K#, Parisien M, Dai Q, Diatchenko L, Pan T. N6-methyladenosine alters RNA structure to regulate binding of a low-complexity protein. Nucleic Acids Research 45 (10), 6051-6063 (2017).

4. Zhou K, Liu N, Pan T. Identification of N6-methyladenosine reader proteins. Methods 126, 105-111 (2017).

5. Liu N*, Pan T.* N6-methyladenosine-coded RNA Epigenetics. Nature Structural & Molecular Biology 23, 98-102 (2016).

6. Zhou K, Parisien M, Dai Q, Liu N, Diatchenko L, Sachleben J, Pan T. N6-Methyladenosine Modification in a Long Noncoding RNA Hairpin Predisposes Its Conformation to Protein Binding. J. Mol. Biol. 428, 822-33 (2016).

7. Liu N, Dai Q, Zheng G, He C, Parisien M, Pan T. N6-methyladenosine-dependent RNA structural switches regulate RNA–protein interactions. Nature 518, 560-564 (2015).

8. Liu N, Pan T. Probing RNA modification status at single nucleotide resolution in messenger and long non-coding RNA. Methods in Enzymology 560, 149-159 (2015).

9. Liu N, Pan T. RNA epigenetics. Transl. Res. 165, 28-35 (2015)

10. Chen K, Lu Z, Wang X, Fu Y, Luo G, Liu N, Han D, Dominissini D, Dai Q, Pan T, He C. High-Resolution N6-Methyladenosine (m6A) Map Using Photo-Crosslinking-Assisted m6A Sequencing. Angew. Chem. Int. Ed. 53, 1-5 (2014).

11. Liu N, Parisien M, Dai Q, Zheng G, He C, Pan T. Probing N6-methyl-adenosine RNA modification status at single nucleotide resolution in mRNA and long noncoding RNA. RNA 19, 1848-1856 (2013).