联系方式

邮箱:chunlai@tsinghua.edu.cn

陈春来 博士

副教授

个人履历

1999-2003北京大学化学与分子工程学院 学士

2003-2008北京大学化学与分子工程学院 博士

2008-2015宾夕法尼亚大学 博士后

2015-2018清华大学生命科学学院 助理教授

2018-至今清华大学生命科学学院 副教授

主要科研领域与方向

1. 发展单分子荧光方法和技术;

2. 核糖体在蛋白质合成过程中翻译和调控机制的研究;

3. CRISPR系列蛋白靶向和剪切机制的研究。

代表性论文

1. Xiaolin Niu(#), Ruirui Sun(#), Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen (*) and Xianyang Fang (*), (2021) Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Nat Commun, 10.1038/s41467-021-26616-x

2. Mengyi Yang, Ruirui Sun, Pujuan Deng, Yuzhuo Yang, Wenjuan Wang, Jun-Jie Gogo Liu (*) and Chunlai Chen (*), (2021) Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chem Sci, 12, 12776-12784

3. Ming-Jie Han(#), Qing-tao He(#), Mengyi Yang(#), Chao Chen, Yirong Yao, Xiaohong Liu, Yuchuan Wang, Zhong-liang Zhu, Kong-kai Zhu, Changxiu Qu, Fan Yang, Cheng Hu, Xuzhen Guo, Dawei Zhang, Chunlai Chen (*), Jin-peng Sun (*) and Jiangyun Wang (*), (2021) Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chem Sci,12, 9114-9123

4. Sijia Peng (#), Weiping Li (#), Yirong Yao (#), Wenjing Xing, Pilong Li (*) and  Chunlai Chen (*), (2020) Phase separation at the nanoscale quantified by dcFCCS, PNAS,117(44):27124-27131

5. Sijia Peng (#), Xiaowei Wang (#), Lu Zhang (#)(*), Shanshan He, Xin Sheng Zhao (*), Xuhui Huang (*) and Chunlai Chen (*), (2020) Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, PNAS,117(36):21889-21895

6. Bo Wu, Haibo Zhang, Ruirui Sun, Sijia Peng, Barry S Cooperman, Yale E Goldman(*), Chunlai Chen(*). (2018) Translocation kinetics and structural dynamics of ribosomes are modulated by the conformational plasticity of downstream pseudoknots. Nucleic Acids Research,46(18): 9736-9748.

7. Sijia Peng, Wenjuan Wang, and Chunlai Chen(*), (2018) Surface Transient Binding-Based Fluorescence Correlation Spectroscopy (STB-FCS), a Simple and Easy-to-Implement Method to Extend the Upper Limit of the Time Window to Seconds,J. Phys. Chem. B,122(18): 4844-4850

8. Mengyi Yang(#), Sijia Peng(#), Ruirui Sun, Jingdi Lin, Nan Wang, and Chunlai Chen(*), (2018) The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET, Cell Reports,22(2): 372-382

9. Sijia Peng, Mengyi Yang, Rui Ning Sun, Yang Liu, Wenjuan Wang, Qiaoran Xi, Haipeng Gong, and Chunlai Chen(*), (2018) Mechanism of actions of Oncocin, a proline-rich antimicrobial peptide, in early elongation revealed by single-molecule FRET, Protein & Cell, 9(10): 890-895

10. Sijia Peng, Ruirui Sun, Wenjuan Wang and Chunlai Chen(*), (2017) Single-Molecule Photoactivation FRET: A General and Easy-to-Implement Approach to Break the Concentration Barrier, Angew. Chem. Int. Ed, 56(24):6882-6885