Cyclic Peptide DataBank (CPDB)

Summary

PDB ID	2BCD Go to RCSB ➚
Title	X-ray crystal structure of Protein Phosphatase-1 with the marine toxin motuporin bound
Method	X-RAY DIFFRACTION
Resolution	2.10 Å
R-factors	R-work: 0.221, R-free: 0.264
Release Date	2006-01-17
Authors	Maynes, J.T.; Luu, H.A.; Cherney, M.M.; Andersen, R.J.; Williams, D.; Holmes, C.F.; James, M.N.
Source Organism	Homo sapiens, marine sponge Thenonella swinhoie grey
Expression Host	Escherichia coli
Gene Names	PPP1CC
Keywords	Protein phosphtase, natural product inhibitors, motuporin, nodularin, HYDROLASE, HYDROLASE-HYDROLASE INHIBITOR COMPLEX
EC Number	3.1.3.16
Molecular Weight	38.41 kDa
Entity Count	Protein: 2, Nucleic Acid: 0, NA Hybrid: 0
Space Group	P 42 21 2
Cell Parameters	a=100.95 Å, b=100.95 Å, c=63.48 Å, α=90.00°, β=90.00°, γ=90.00°
Other Links	Yes
Peptide Chains	B
Protein Chains	A
Peptide Lengths	5
Protein Lengths	323

Sequence Information

Chain: A - Serine/threonine protein phosphatase PP1-gamma catalytic subunit (Length: 323)

1	MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK
61	ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL
121	LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL
181	QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD
241	LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE
301	KKKPNATRPV TPPRGMITKQ AKK

Chain: B - MOTUPORIN (Length: 5)

1

DVXEX

References

Crystal Structures of Protein Phosphatase-1 Bound to Motuporin and Dihydromicrocystin-LA: Elucidation of the Mechanism of Enzyme Inhibition by Cyanobacterial Toxins.

Maynes, J.T., Luu, H.A., Cherney, M.M., Andersen, R.J., Williams, D., Holmes, C.F., James, M.N.

J Mol Biology, 2006

PubMed: 16343532 | DOI: 10.1016/j.jmb.2005.11.019

Ligand Information

ID	Name	Formula	Molecular Weight
BME	BETA-MERCAPTOETHANOL	C2 H6 O S	78.133
MN	MANGANESE (II) ION	Mn	54.938

Biological Assemblies

Assembly ID: 1

Details: author_defined_assembly

Oligomeric State: Monomer

Stoichiometry: A1