Cyclic Peptide DataBank (CPDB)

Summary

PDB ID	2VUU Go to RCSB ➚
Title	Crystal structure of NADP-bound NmrA-AreA zinc finger complex
Method	X-RAY DIFFRACTION
Resolution	2.80 Å
R-factors	R-work: 0.232, R-free: 0.287
Release Date	2008-07-29
Authors	Kotaka, M.; Johnson, C.; Lamb, H.K.; Hawkins, A.R.; Ren, J.; Stammers, D.K.
Source Organism	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)
Expression Host	Escherichia coli BL21, Escherichia coli BL21(DE3)
Keywords	TRANSCRIPTION REGULATION, PROTEIN-PROTEIN INTERACTIONS, METAL-BINDING, NITRATE ASSIMILATION, ZINC-FINGER, DNA-BINDING, ZINC FINGERS, TRANSCRIPTION, ZINC, AREA, NMRA, NUCLEUS, ACTIVATOR, GATA-TYPE
Molecular Weight	355.47 kDa
Entity Count	Protein: 2, Nucleic Acid: 0, NA Hybrid: 0
Space Group	H 3
Cell Parameters	a=231.73 Å, b=231.73 Å, c=223.45 Å, α=90.00°, β=90.00°, γ=120.00°
Disulfide Bond	K676-K694;L694-L697
Other Links	Yes
Peptide Chains	I, J, K, L, M, N, O, P
Protein Chains	A, B, C, D, E, F, G, H
Peptide Lengths	43
Protein Lengths	352

1	MAQQKKTIAV VNATGRQAAS LIRVAAAVGH HVRAQVHSLK GLIAEELQAI PNVTLFQGPL
61	LNNVPLMDTL FEGAHLAFIN TTSQAGDEIA IGKDLADAAK RAGTIQHYIY SSMPDHSLYG
121	PWPAVPMWAP KFTVENYVRQ LGLPSTFVYA GIYNNNFTSL PYPLFQMELM PDGTFEWHAP
181	FDPDIPLPWL DAEHDVGPAL LQIFKDGPQK WNGHRIALTF ETLSPVQVCA AFSRALNRRV
241	TYVQVPKVEI KVNIPVGYRE QLEAIEVVFG EHKAPYFPLP EFSRPAAGSP KGLGPANGKG
301	AGAGMMQGPG GVISQRVTDE ARKLWSGWRD MEEYAREVFP IEEEANGLDW ML

1	PTTCTNCFTQ TTPLWRRNPE GQPLCNACGL FLKLHGVVRP LSL

Structural Analysis of the Recognition of the Negative Regulator Nmra and DNA by the Zinc Finger from the Gata-Type Transcription Factor Area.

Kotaka, M., Johnson, C., Lamb, H.K., Hawkins, A.R., Ren, J., Stammers, D.K.

J Mol Biology, 2008

ID	Name	Formula	Molecular Weight
NAP	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	C21 H28 N7 O17 P3	743.405
ZN	ZINC ION	Zn	65.409

Assembly ID: 1

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 2

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 3

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 4

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 5

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 6

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 7

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 8

Details: software_defined_assembly

Oligomeric State: Hetero 2-mer

Stoichiometry: A1, B1

Assembly ID: 9