Cyclic Peptide DataBank (CPDB)

Sequence Information

Chain: A - DNA-DIRECTED RNA POLYMERASE (Length: 880)

1	MSEKNIKGIK FGILSPDEIR KMSVTAIITP DVYDEDGTPI EGSVMDPRLG VIEPGQKCPT
61	CGNTLGNCPG HFGHIELVRP VIHVGFVKHV YEFLKATCRR CGRVKISEDE IEKYSRIYNA
121	IKKRWPSAAR RLTEYVKKTA MKAQVCPHCG EKQFKIKLEK PYNFYEERKE GVAKLTPSDI
181	RERLEKVPES DVEILGYDPT TSRPEWMILT VLPVPPITIR PSIMIESGIR AEDDLTHKLV
241	DIVRINERLK ESIDAGAPQL IIEDLWDLLQ YHVATYFDNE IPGLPPSKHR SGRPLRTLAQ
301	RLKGKEGRFR GNLSGKRVDF SSRTVISPDP NISIDEVGVP EIIARTLTVP ERITPWNIEK
361	LRQFVINGPD KWPGANYVIR PDGRRIDLRY VKDRKELAST LAPGYVVERH LTDGDVVLFN
421	RQPSLHRISM MAHRVRVLKG LTFRLNLLVC PPYNADFDGD EMNLHVPQSE EAIAEAKEIM
481	LVHKNIITPR YGGPIIGAAQ DYISGAYLLT VKTTLLTKEE AQQILGVADV KIDLGEPAIL
541	APREYYTGKQ VVSAFLPKDF NFHGQANVSS GPRLCKNEDC PHDSYVVIKN GILLEGVFDK
601	KAIGNQQPES ILHWLIKEYS DEYGKWLMDN LFRVFIRFVE LQGFTMRLED VSLGDDVKKE
661	IYNEIDRAKV EVDNLIQKYK NGELEPIPGR TLEESLENYI LDTLDKLRST AGDIASKYLD
721	PFNFAYVMAR TGARGSVLNI TQMAAMLGQQ SVRGERIKRG YMTRTLPHFK PYDISPEARG
781	FIYSSFRTGL KPTELFFHAA GGREGLVDTA VRTSQSGYMQ RRLINALSDL RAEYDGTVRS
841	LYGEVIQVAY GDDGVFPMYS AHGKTVDVNR IFERVVGWKT

Chain: L - DNA-DIRECTED RNA POLYMERASE (Length: 92)

1	MEIKILKSES NYLELEIEGE DHTLGNLIAG TLRKISGVSF ASYYQPHPLT DKIIVKILTD
61	GSIAPKDALL KAIETVRVMA SHYIDEIKGL TK

Chain: N - DNA-DIRECTED RNA POLYMERASE (Length: 66)

1	MMIPIRCFTC GSLIADKWQP FITRVNAGEN PGKVLDDLGV KRYCCRRMLL SHIDIISEVI
61	HYTRPI

Chain: P - DNA-DIRECTED RNA POLYMERASE (Length: 48)

1	MAVYRCGKCW KTFTDEQLKV LPGVRCPYCG YKIIFMVRKP TIKIVKAI

Chain: Q - DNA-DIRECTED RNA POLYMERASE (Length: 104)

1	MVSGMSTEEE KEGTNDEEVS EEREVEETSE EEFPKLSIQD IELLMKNTEI WDNLLNGKIS
61	VDEAKRLFED NYKDYEKRDS RRKAKKAASK KVKKTKKKEK SVEG

Chain: B - DNA-DIRECTED RNA POLYMERASE (Length: 1131)

1	MNELSSNLSI DERWKVIEAY FKSKGLVRQH LDSYNDFVRN KLQEIIDEQG EIPTEIPGLK
61	VRLGKIRIGK PRVRESDRGE REISPMEARL RNLTYAAPLW LTMIPVENNI EAEPEEVYIG
121	DLPIMLKSAI DPISQYTLDK LIEIGEDPKD PGGYFIVNGS ERVIVTQEDL APNRVLVDTG
181	KTGSNITHTA KIISSTAGYR VPVTIERLKD GTFHVSFPAV PGKIPFVILM RALGILTDRD
241	IVYAVSLDPE IQNELFPSLE QASSIANVDD ALDFIGSRVA IGQKRENRIE KAQQIIDKYF
301	LPHLGTSADD RRKKAYYLAY AISKVIELYL GRREPDDKDH YANKRLRLAG DLFASLFRVA
361	FKAFVKDLTY QLEKSKVRGR KLALKALVRP DIVTERIRHA LATGNWVGGR TGVSQLLDRT
421	NWLSMLSHLR RVISSLARGQ PNFEARDLHG TQWGRMCPFE TPEGPNSGLV KNLALMAQIA
481	VGINEKIVEK TLYEMGVVPV EEVIRRVTEG GEDQNEYLKW SKVILNGRLV GYYRDGEELA
541	KKIRERRRKG EISDEVNVGH IVTDFINEVH VNCDSGRVRR PLIIVSNGNP LVTREDIEKL
601	DSGSITFDDL VRQGKIEYLD AEEEENAYVA LEPSDLTPEH THLEIWSPAI LGITASIIPY
661	PEHNQSPRNT YQSAMAKQAL GLYAANYQLR TDTRAHLLHY PQRPLVQTRA LDIIGYTNRP
721	AGNNAILAVI SFTGYNMEDS IIMNRSSVER GMYRSTFFRL YSTEEVKYPG GQEDKIVMPE
781	PGVRGYKGKE YYRLLEDNGV VSPEVEVKGG DVLIGKVSPP RFLQEFKELS PEQAKRDTSI
841	VTRHGEMGIV DLVLITETAE GNKLVKVRVR DLRIPSIGDK FASRHGQKGV IGMLIPQVDM
901	PYTVKGVVPD VILNPHALPS RMTLGQIMEG IAGKYAALSG NIVDATPFYK TPIEQLQNEI
961	LKYGYLPDAT EVTYDGRTGQ KIKSRIYFGV VYYQKLHHMV ADKIHARARG PVQILTRQPT
1021	EGRAREGGLR FGEMERDCLI GFGTAMLLKD RLLDNSDRTT IYVCDQCGYI GWYDKNKNKY
1081	VCPIHGDKSN LFPVTVSYAF KLLIQELMSM IISPRLILED RVGLSGGKGN E

Chain: C - DNA-DIRECTED RNA POLYMERASE (Length: 395)

1	MENVIDEKDK SYLEEKVKQA SNILPQKIVE DLKNLISNKE VLVTRDEIDK IFDLAIKEYS
61	EGLIAPGEAI GIVAAQSVGE PGTQMTLRTF HFAGIRELNV TLGLPRLIEI VDAKKVPSTP
121	MMTIYLTDEY KHDKEKALEV ARKLEYTKIE NVVSSTSIDI ASMSIILQLD NEMLKDKGVT
181	VDDVKKAINR LKLGEFVIDE SEGNTLNISF ANIDSIAALF KLRDKILNTK IKGIKGIKRA
241	IVQKKGDEYI ILTDGSNLSG VLSVKGVDIA KVETNNIREI EEVFGIEAAR EIIIREISKV
301	LAEQGLDVDM RHILLVADVM TRTGVVRQIG RHGVTGEKNS VLARAAFEVT VKHLLDAAAR
361	GDVEEFKGVV ENIIIGHPIK LGTGMVELTM RPILR

Chain: D - DNA-DIRECTED RNA POLYMERASE (Length: 265)

1	MSINLLHKDD KRIDLVFEGY PLEFVNAIRR AAMLYVPVMS IDDVYFIENN SPLYDEILAH
61	RLALIPFTSE EALDTYRWPE ECIDCTENCE KCYTKIYIEA EALNEPKMLY SKDIKSEDPS
121	IVPISGDIPI VLLGANQKIS LEARLRLGYG KEHAKFIPVS LAIVRYYPKV EILGNCEKGA
181	TVCPEGVFEL KDGKLSVKNE LACTLCEECL RYCNGLIRIS SVEDKYILEL ESVGSLKPER
241	ILLEAGKSII RKIEELEKKL VEVIK

Chain: E - DNA-DIRECTED RNA POLYMERASE (Length: 180)

1	MYKLIKARSI VRIPPNEFGK PLNEIALNEL RQQYQEKILK DLGLVLAILN VKTSEEGMLV
61	FGDGATYHEV EFDMITYVPV VQEVVEGEVL QVDNYGVFVN LGPMDGLVHI SQITDDTLKY
121	DNVRGIIFGE KSKKVIQKGD KVRARVISVA STVTGRLPRI ALTMRQPYLG KLEWITQAKK

Chain: F - DNA-DIRECTED RNA POLYMERASE (Length: 113)

1	MSSVYIVEEH YIPYSVAKKL LSDVIKSGSS SNLLQRTYDY LNSVEKCDAE SAQKVVEELS
61	SIISREDVRA VLASICPITP DEVRSILIMD SNRTYTSEDI QKIIDIIRKY IKS

Chain: G - DNA-DIRECTED RNA POLYMERASE (Length: 132)

1	MMESKAQEII LSCEINSIER GSLKNLSIIH MSCNDFNISF DIIDSINIFS QKEKVKAFIS
61	KNRLSYTNDD FCGHGYIVTE LKDSSSNNGN RYITIISLFG LLVKIISNKE SFLKIHQLNV
121	MDHIYFCVKK NT

Chain: H - DNA-DIRECTED RNA POLYMERASE (Length: 84)

1	MRGSSNRKID PRIHYLVPKH EVLSIDEAYK ILKELGIRPE QLPWIRASDP VARSINAKPG
61	DIIRIIRKSQ LYGEVVSYRY VISG

Chain: K - DNA-DIRECTED RNA POLYMERASE (Length: 95)

1	MGLERDGILS QDLHFNEVFV SLWQNKLTRY EIARVISARA LQLAMGAPAL IDINNISSTD
61	VISIAEEEFK RGVLPITIRR RLPNGKIILL SLRKS

References

Structural and Functional Analyses of the Interaction of Archaeal RNA Polymerase with DNA.

Wojtas, M.N., Mogni, M., Millet, O., Bell, S.D., Abrescia, N.G.A.

Nucleic Acids Res, 2012

PubMed: 22848102 | DOI: 10.1093/NAR/GKS692

Archaeal RNA Polymerase: The Influence of the Protruding Stalk in Crystal Packing and Preliminary Biophysical Analysis of the Rpo13 Subunit.

Wojtas, M., Peralta, B., Ondiviela, M., Mogni, M., Bell, S.D., Abrescia, N.G.A.

Biochem Soc Trans, 2011

PubMed: 21265742 | DOI: 10.1042/BST0390025

Evolution of Complex RNA Polymerases: The Complete Archaeal RNA Polymerase Structure.

Korkhin, Y., Unligil, U.M., Littlefield, O., Nelson, P.J., Stuart, D.I., Sigler, P.B., Bell, S.D., Abrescia, N.G.

PLoS Biol, 2009

PubMed: 19419240 | DOI: 10.1371/JOURNAL.PBIO.1000102

PDB ID	4AYB Go to RCSB ➚
Title	RNAP at 3.2Ang
Method	X-RAY DIFFRACTION
Resolution	3.20 Å
R-factors	R-work: 0.242, R-free: 0.300
Release Date	2012-08-08
Authors	Wojtas, M.N.; Mogni, M.; Millet, O.; Bell, S.D.; Abrescia, N.G.A.
Source Organism	SULFOLOBUS SHIBATAE
Keywords	TRANSFERASE, MULTI-SUBUNIT, TRANSCRIPTION
EC Number	2.7.7.6
Molecular Weight	406.67 kDa
Entity Count	Protein: 13, Nucleic Acid: 0, NA Hybrid: 0
Space Group	P 21 21 2
Cell Parameters	a=195.72 Å, b=212.41 Å, c=128.76 Å, α=90.00°, β=90.00°, γ=90.00°
Disulfide Bond	P9-P26
Other Links	Yes
Peptide Chains	P
Protein Chains	A, B, C, D, E, F, G, H, K, L, N, Q
Peptide Lengths	48
Protein Lengths	880, 92, 66, 104, 1131, 395, 265, 180, 113, 132, 84, 95

ID	Name	Formula	Molecular Weight
ZN	ZINC ION	Zn	65.409
MG	MAGNESIUM ION	Mg	24.305
SF4	IRON/SULFUR CLUSTER	Fe4 S4	351.64