Cyclic Peptide DataBank (CPDB)

Summary

PDB ID	5BQQ Go to RCSB ➚
Title	Human insulin with intra-chain chemical crosslink between modified B27 and B30
Method	X-RAY DIFFRACTION
Resolution	1.54 Å
R-factors	R-work: 0.161, R-free: 0.195
Release Date	2016-02-03
Authors	Brzozowski, A.M.; Turkenburg, J.P.; Jiracek, J.; Zakova, L.
Source Organism	Homo sapiens
Keywords	Chemical crosslink, B24-B29, specificity, hormone
Molecular Weight	35.83 kDa
Entity Count	Protein: 2, Nucleic Acid: 0, NA Hybrid: 0
Space Group	P 31
Cell Parameters	a=60.99 Å, b=60.99 Å, c=81.92 Å, α=90.00°, β=90.00°, γ=120.00°
Disulfide Bond	A6-A11;C6-C11;E6-E11;G6-G11;I6-I11;K6-K11
Other Links	Yes
Peptide Chains	A, B, C, D, E, F, G, H, I, J, K, L
Peptide Lengths	21, 30

Sequence Information

Chain: A, C, E, G, I, K - Insulin (Length: 21)

1	GIVEQCCTSI CSLYQLENYC N

Chain: B, D, F, H, J, L - Insulin (Length: 30)

1	FVNQHLCGSH LVEALYLVCG ERGFFYVPGX

References

Rational steering of insulin binding specificity by intra-chain chemical crosslinking.

Vikova, J., Collinsova, M., Kletvikova, E., Budesinsky, M., Kaplan, V., Zakova, L., Veverka, V., Hexnerova, R., Avino, R.J., Strakova, J., Selicharova, I., Vanek, V., Wright, D.W., Watson, C.J., Turkenburg, J.P., Brzozowski, A.M., Jiracek, J.

Sci Rep, 2016

PubMed: 26792393 | DOI: 10.1038/srep19431

Ligand Information

ID	Name	Formula	Molecular Weight
IPH	PHENOL	C6 H6 O	94.111
ZN	ZINC ION	Zn	65.409
CL	CHLORIDE ION	Cl	35.453

Biological Assemblies

Assembly ID: 1

Details: software_defined_assembly

Oligomeric State: Hetero 12-mer

Stoichiometry: A6, B6