6E3Y - Cyclic Peptide Structure

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Summary

PDB ID	6E3Y Go to RCSB ➚
Title	Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
Method	ELECTRON MICROSCOPY
Resolution	3.30 Å
Release Date	2018-09-19
Authors	Liang, Y.L.; Khoshouei, M.; Deganutti, G.; Glukhova, A.; Koole, C.; Peat, T.S.; Radjainia, M.; Plitzko, J.M.; Baumeister, W.; Miller, L.J.; Hay, D.L.; Christopoulos, A.; Reynolds, C.A.; Wootten, D.; Sexton, P.M.
Source Organism	Homo sapiens, Lama glama
Expression Host	Escherichia coli, Trichoplusia ni
Gene Names	CALCRL, CGRPR, GNAS, GNAS1, GNB1, GNG2, GSP, RAMP1
Keywords	class B G protein-coupled receptor, agonist-receptor-G protein ternary complex, calcitonin gene-related peptide receptor, receptor activity modifying protein 1, active-state G protein-coupled receptor, signaling protein, phase plate, phase contrast
EC Number	3.6.5
Molecular Weight	184.36 kDa
Entity Count	Protein: 7, Nucleic Acid: 0, NA Hybrid: 0
Disulfide Bond	P2-P7
Other Links	Yes
Peptide Chains	P
Protein Chains	A, B, E, G, N, R
Peptide Lengths	38
Protein Lengths	138, 149, 394, 350, 71, 490

Sequence Information

Chain: N - Nanobody 35 (Length: 138)

1	QVQLQESGGG LVQPGGSLRL SCAASGFTFS NYKMNWVRQA PGKGLEWVSD ISQSGASISY
61	TGSVKGRFTI SRDNAKNTLY LQMNSLKPED TAVYYCARCP APFTRDCFDV TSTTYAYRGQ
121	GTQVTVSSHH HHHHEPEA

Chain: E - Receptor activity-modifying protein 1 (Length: 149)

1	MKTIIALSYI FCLVFADYKD DDDKHGSCQE ANYGALLREL CLTQFQVDME AVGETLWCDW
61	GRTIRSYREL ADCTWHMAEK LGCFWPNAEV DRFFLAVHGR YFRSCPISGR AVRDPPGSIL
121	YPFIVVPITV TLLVTALVVW QSKRTEGIV

Chain: P - Calcitonin gene-related peptide 1 (Length: 38)

1	ACDTATCVTH RLAGLLSRSG GVVKNNFVPT NVGSKAFX

Chain: A - Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Length: 394)

1	MGCLGNSKTE DQRNEEKAQR EANKKIEKQL QKDKQVYRAT HRLLLLGAGE SGKNTIVKQM
61	RILHVNGFNG EGGEEDPQAA RSNSDGEKAT KVQDIKNNLK EAIETIVAAM SNLVPPVELA
121	NPENQFRVDY ILSVMNVPDF DFPPEFYEHA KALWEDEGVR ACYERSNEYQ LIDCAQYFLD
181	KIDVIKQADY VPSDQDLLRC RVLTSGIFET KFQVDKVNFH MFDVGAQRDE RRKWIQCFND
241	VTAIIFVVAS SSYNMVIRED NQTNRLQAAL KLFDSIWNNK WLRDTSVILF LNKQDLLAEK
301	VLAGKSKIED YFPEFARYTT PEDATPEPGE DPRVTRAKYF IRDEFLRIST ASGDGRHYCY
361	PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ YELL

Chain: B - Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (Length: 350)

1	MHHHHHHGSS GSELDQLRQE AEQLKNQIRD ARKACADATL SQITNNIDPV GRIQMRTRRT
61	LRGHLAKIYA MHWGTDSRLL VSASQDGKLI IWDSYTTNKV HAIPLRSSWV MTCAYAPSGN
121	YVACGGLDNI CSIYNLKTRE GNVRVSRELA GHTGYLSCCR FLDDNQIVTS SGDTTCALWD
181	IETGQQTTTF TGHTGDVMSL SLAPDTRLFV SGACDASAKL WDVREGMCRQ TFTGHESDIN
241	AICFFPNGNA FATGSDDATC RLFDLRADQE LMTYSHDNII CGITSVSFSK SGRLLLAGYD
301	DFNCNVWDAL KADRAGVLAG HDNRVSCLGV TDDGMAVATG SWDSFLKIWN

Chain: G - Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (Length: 71)

1	MASNNTASIA QARKLVEQLK MEANIDRIKV SKAAADLMAY CEAHAKEDPL LTPVPASENP
61	FREKKFFCAI L

Chain: R - Calcitonin gene-related peptide type 1 receptor (Length: 490)

1	MKTIIALSYI FCLVFADYKD DDDLEVLFQG PAELEESPED SIQLGVTRNK IMTAQYECYQ
61	KIMQDPIQQA EGVYCNRTWD GWLCWNDVAA GTESMQLCPD YFQDFDPSEK VTKICDQDGN
121	WFRHPASNRT WTNYTQCNVN THEKVKTALN LFYLTIIGHG LSIASLLISL GIFFYFKSLS
181	CQRITLHKNL FFSFVCNSVV TIIHLTAVAN NQALVATNPV SCKVSQFIHL YLMGCNYFWM
241	LCEGIYLHTL IVVAVFAEKQ HLMWYYFLGW GFPLIPACIH AIARSLYYND NCWISSDTHL
301	LYIIHGPICA ALLVNLFFLL NIVRVLITKL KVTHQAESNL YMKAVRATLI LVPLLGIEFV
361	LIPWRPEGKI AEEVYDYIMH ILMHFQGLLV STIFCFFNGE VQAILRRNWN QYKIQFGNSF
421	SNSEALRSAS YTVSTISDGP GYSHDCPSEH LNGKSIHDIE NVLLKPENLY NPAGLEVLFQ
481	GPHHHHHHHH

References

Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor.

Liang, Y.L., Khoshouei, M., Deganutti, G., Glukhova, A., Koole, C., Peat, T.S., Radjainia, M., Plitzko, J.M., Baumeister, W., Miller, L.J., Hay, D.L., Christopoulos, A., Reynolds, C.A., Wootten, D., Sexton, P.M.

Nature, 2018

PubMed: 30209400 | DOI: 10.1038/s41586-018-0535-y

Biological Assemblies

Assembly ID: 1

Details: author_defined_assembly

Oligomeric State: Hetero 7-mer

Stoichiometry: A1, B1, C1, D1, E1, F1, G1

Cyclic Peptide DataBank (CPDB)