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Cyclic Peptide DataBank (CPDB)

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7URE - Cyclic Peptide Structure

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Summary

PDB ID7URE Go to RCSB ➚
TitleHuman PORCN in complex with palmitoleoylated WNT3A peptide
MethodELECTRON MICROSCOPY
Resolution3.19 Å
Release Date2022-07-20
AuthorsLiu, Y.; Qi, X.; Li, X.
Source OrganismHomo sapiens, Mus musculus
Expression HostHomo sapiens
Gene NamesMG61, PORC, PORCN, PPN
Keywordsproduct-bound, complex, TRANSFERASE, TRANSFERASE-TRANSFERASE PRODUCT complex, TRANSFERASE-TRANSFERASE INHIBITOR complex
EC Number2.3.1.250
Molecular Weight109.51 kDa
Entity CountProtein: 4, Nucleic Acid: 0, NA Hybrid: 0
Disulfide BondB203-B217;B205-B212
Other LinksYes
Peptide ChainsB
Protein ChainsA, H, L
Peptide Lengths22
Protein Lengths464, 234, 250

Sequence Information

Chain: A - Isoform 2 of Protein-serine O-palmitoleoyltransferase porcupine (Length: 464)

1MDYKDDDDKA TFSRQEFFQQ LLQGCLLPTA QQGLDQIWLL LAICLACRLL WRLGLPSYLK
61HASTVAGGFF SLYHFFQLHM VWVVLLSLLC YLVLFLCRHS SHRGVFLSVT ILIYLLMGEM
121HMVDTVTWHK MRGAQMIVAM KAVSLGFDLD RGEVGTVPSP VEFMGYLYFV GTIVFGPWIS
181FHSYLQAVQG RPLSCRWLQK VARSLALALL CLVLSTCVGP YLFPYFIPLN GDRLLRNKKR
241KARWLRAYES AVSFHFSNYF VGFLSEATAT LAGAGFTEEK DHLEWDLTVS KPLNVELPRS
301MVEVVTSWNL PMSYWLNNYV FKNALRLGTF SAVLVTYAAS ALLHGFSFHL AAVLLSLAFI
361TYVEHVLRKR LARILSACVL SKRCPPDCSH QHRLGLGVRA LNLLFGALAI FHLAYLGSLF
421DVDVDDTTEE QGYGMAYTVH KWSELSWASH WVTFGCWIFY RLIG

Chain: L - 2C11 light chain (Length: 234)

1MGWSCIILFL VATARTGVHS DIHMTQSPAS LSAFVGETVT ITCRTSENIF SYLAWYQQKQ
61GKSPQLLVYN AKTLTSGVPS RFSGSGSGTQ FSLKINSLQP EDFGSYYCQH HYGSPYTFGG
121GTKLEIKRTV AAPSVFIFPP SDEQLKSGTA SVVCLLNNFY PREAKVQWKV DNALQSGNSQ
181ESVTEQDSKD STYSLSSTLT LSKADYEKHK VYACEVTHQG LSSPVTKSFN RGEC

Chain: H - 2C11 heavy chain (Length: 250)

1MGWSCIILFL VATATGVHSE IQLQQSGAEL VKPGASVKMS CKVSGYSFTG YNMNWVKQSH
61GKSLEWIGNI NPYYVSTNYN QKFTGKATFT VDRSSSTAYM QLDSLTSEDS AVYYCARSYG
121SSHTFAYWGQ GTLVTVSSAS TKGPSVFPLA PSSKSTSGGT AALGCLVKDY FPEPVTVSWN
181SGALTSGVHT FPAVLQSSGL YSLSSVVTVP SSSLGTQTYI CNVNHKPSNT KVDKRVEPKS
241CDKTHHHHHH

Chain: B - Protein Wnt-3a (Length: 22)

1MHLKCKCHGL AGSCEVKTCW WS

References

Mechanisms and inhibition of Porcupine-mediated Wnt acylation.

Liu, Y., Qi, X., Donnelly, L., Elghobashi-Meinhardt, N., Long, T., Zhou, R.W., Sun, Y., Wang, B., Li, X.

Nature, 2022

PubMed: 35831507 | DOI: 10.1038/s41586-022-04952-2

Ligand Information

ID Name Formula Molecular Weight
AJPDigitoninC56 H92 O291229.312
ZNZINC IONZn65.409
PAMPALMITOLEIC ACIDC16 H30 O2254.408

Biological Assemblies

Assembly ID: 1

Details: author_defined_assembly

Oligomeric State: Hetero 4-mer

Stoichiometry: A1, B1, C1, D1

3D Structure Visualization