Cyclic Peptide DataBank (CPDB)

Summary

PDB ID	8H8Q Go to RCSB ➚
Title	Fab-amyloid beta fragment complex at neutral pH
Method	X-RAY DIFFRACTION
Resolution	2.50 Å
R-factors	R-work: 0.212, R-free: 0.275
Release Date	2023-10-25
Authors	Kita, A.; Irie, K.; Irie, Y.; Matsushima, Y.; Miki, K.
Source Organism	Homo sapiens, Mus musculus
Expression Host	Mus musculus
Keywords	FAB COMPLEX, IMMUNE SYSTEM
Molecular Weight	49.54 kDa
Entity Count	Protein: 3, Nucleic Acid: 0, NA Hybrid: 0
Space Group	P 21 21 21
Cell Parameters	a=40.04 Å, b=94.15 Å, c=115.44 Å, α=90.00°, β=90.00°, γ=90.00°
Disulfide Bond	A17-A28
Other Links	Yes
Peptide Chains	A
Protein Chains	H, L
Peptide Lengths	15
Protein Lengths	218, 220

Sequence Information

Chain: L - Fab (Length: 218)

1	DIVMTQSPSS LALSVGQKVT MNCKSSQSLL NSDNQKNYLA WYQQKPGQSP KLLIYFASTR
61	ESGVPDRFMG SGSGTDFNLS ISSVQPEDLA DYFCQQHYSP PLSFGAGTRL ELKRADAAPT
121	VSIFPPSSEQ LTSGGASVVC FLNNFYPKDI NVKWKIDGSE RQNGVLNSWT DQDSKDSTYS
181	MSSTLTLTKD EYERHNSYTC EATHKTSTSP IVKSFNRN

Chain: H - Fab (Length: 220)

1	QVQLQQPGAE LVRPGSSVKL SCRASGYTFT SYWVSWVQQR PGQGLEWIGM IHPSDGEARL
61	NQKFKDKATL TVDKSSTTVY MQLSSPTSED SAVYYCALFD GYYPWFASWG QGTLVTVSAA
121	KTTPPSVYPL APGSAAQTNS MVTLGCLVKG YFPEPVTVTW NSGSLSSGVH TFPAVLQSDL
181	YTLSSSVTVP SSTWPSETVT CNVAHPASST KVDKKIVPRD

Chain: A - GLN-LYS-CYS-VAL-PHE-PHE-ALA-GLU-ASP-VAL-GLY-SER-ASN-CYS-GLY (Length: 15)

1	QKCVFFAEDV GSNCG

References

Identification of the binding site and immunoreactivity of anti-A beta antibody 11A1: Comparison with the toxic conformation-specific TxCo-1 antibody.

Fukui, R., Hafizal, U., Kageyama, Y., Irie, Y., Matsushima, Y., Hosoi, K., Nakayama, T., Kaneda, D., Hashizume, Y., Miki, K., Kita, A., Mukaisho, K.I., Kushima, R., Tooyama, I., Irie, K.

Biochem Biophys Res Commun, 2025

PubMed: 40120343 | DOI: 10.1016/j.bbrc.2025.151655

Ligand Information

ID	Name	Formula	Molecular Weight
CL	CHLORIDE ION	Cl	35.453
NA	SODIUM ION	Na	22.99

Biological Assemblies

Assembly ID: 1

Details: author_defined_assembly

Oligomeric State: Hetero 3-mer

Stoichiometry: A1, B1, C1