Guangshuo Ou Ph.D.

Main Research Fields

1. Our laboratory studies how cytoskeleton and signaling-related biomacromolecules regulate cell-cell fusion using Caenorhabditis elegans as the model system.

2. We have developed live time-lapse fluorescence imaging techniques to visualize cell-cell fusion and neural development by spinning disk confocal microscopy. With the combination of our live cell microscopy and powerful genetic engineering methodologies, we expect to better understand molecular mechanisms of cell dynamics in C. elegans.

Selected Publications

1. RNA editing restricts hyperactive ciliary kinases. Li D, Liu Y, Yi P, Zhu Z, Li W, Zhang QC, Li JB, Ou G*. Science. 2021 Aug 27;373(6558):984-991. doi: 10.1126/science.abd8971.

2. Zhu Z, Chai Y, Hu H, Li W, Li WJ, Dong MQ, Wu JW, Wang ZX, Ou G*. (2020)Spatial Confinement of Receptor Activity by Tyrosine Phosphatase During Directional Cell Migration. Proc Natl Acad Sci U S A,117(25):14270-14279.

3. Xie C, Li L, Li M, Shao W, Zuo Q, Huang X, Chen R, Li W, Brunnbauer M, Ökten Z, Chen L *, Ou G *.(2020) Optimal Sidestepping of Intraflagellar Transport Kinesins Regulates Structure and Function of Sensory Cilia. EMBO J,9(12):e103955.

4. Jia R, Li D, Li M, Chai Y, Liu Y, Xie Z, Shao W, Xie C, Li L, Huang X, Chen L, Li W, Ou G.(2019) Spectrin-based membrane skeleton supports ciliogenesis. PLoS Biol,17(7):e3000369.

5. Yang Y, Zhang Y, Li WJ, Jiang Y, Zhu Z, Hu H, Li W, Wu JW, Wang ZX, Dong MQ, Huang S, Ou G. (2017) Spectraplakin Induces Positive Feedback between Fusogens and the Actin Cytoskeleton to Promote Cell-Cell Fusion. Developmental Cell, 41(1):107-120.

6. Feng G, Zhu Z, Li W, Chai Y, Dong M, Ou G. (2017) Hippo Kinases Maintain Polarity during Directional Cell Migration in C. elegans. EMBO J,36(3):334-345.

7. Yi P, Li WJ, Dong MQ, Ou G*.(2017) Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C. elegans Sensory Cilia. Current Biology,27(10):1448-1461.

8. Li W, Yi P, Zhu Z, Zhang X, Li W, Ou G. (2017) Centriole translocation and degeneration during ciliogenesis in Caenorhabditis elegans neurons. EMBO J,36(17):2553-2566.

9. Zhu Z, Chai Y, Jiang Y, Li WJ, Hu H, Li W, Wu J, Wang Z, Huang S, Ou G. (2016) Functional Coordination of WAVE and WASP in C. elegans Neuroblast Migration. Developmental Cell, 39(2):224-238.

10. Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W, Ou G. (2014) Conditional Knockouts Generated by Engineered CRISPR-Cas9 Endonuclease Reveal the Roles of Coronin in C. elegans Neural Development. Developmental Cell,30(5):625-36.