李丕龙 博士

副教授
2021 无锡AppTec生命科学与化学奖获得者
2021国家自然科学基金杰出青年基金
2019拜耳研究员

主要科研领域与方向

真核细胞有许多无脂膜的微米级三维细胞器行使非常重要的功能，但人们还不清楚这些无膜细胞器的形成机理。此前我们发现，多价蛋白和它们的多价配体会发生“液－液分离相变”现象，即产生一个正常的溶液相和一个蛋白富积的粘稠的液体相。这个蛋白富积的液体相的物理学特性与上述的无膜细胞器非常类似。人们研究已知，多价的生物大分子在细胞里普遍存在，尤其在这些无膜细胞器里，由此我们猜想多价介导的“液－液分离相变”很可能是无膜细胞器形成的重要原因之一，并且细胞具有通过特定的翻译后修饰来调节某些系统的“液－液分离相变”的潜能。本实验室致力于研究“液－液分离相变”在细胞生物学上的意义。另外，宏观上的相变是微观下分子互作的体现。所以，我们可以用宏观上的相变与否来反映微观下是否有分子／分子相互作用。我们在开发基于“液－液分离相变”的生化系统，用于在体外或体内研究任意分子互作。

代表性论文

1. He J^#, Huo X^#, Pei G^#, et al., Li Pilong*, and Li Yinqing*. Transcription Factors with Dual Roles as Activators and Repressors Regulate Stable Intermediate Expression Levels. Cell 2024, 187(11):2746.

2. Jia W, Li W, Li Zengpeng*, and Li Pilong*. An all-in-one targeted protein degradation platform guided by a bridging bi-specific nanobody. Cell Research 2024, 34(5):389.

3. Li R, Li T, et al., Wang Y, Du Juanjuan*, and Li Pilong*. Programming cell surface signaling via phase separation-controlled compartmentalization. Nature Chemical Biology 2022 18(12):1351.

4. Wang Y^#, Yu C^#, Pei G^#, Jia W, Li Tingting*, and Li Pilong*. Prevalent aberrant condensates of onco-fusion transcription factors and their dissolution as potential cancer therapy. Nature Chemical Biology 2023, 19(10):1223.

5. Buttress T^#, He S^#, Wang L^#, Zhou S^#, et al., Li Pilong*, and Feng X*. A histone variant condenses flowering plant sperm via chromatin phase separation. Nature 2022 611(7936):614.

6. Xu W^#, Pei G^#, Liu H^#, et al., Li Pilong*. (2021) Compartmentalization-aided interaction screening reveals extensive high-order complexes within the SARS-CoV-2 proteome. Cell Reports 36:109482.

7. Wang L^#, Hu M^#, Zuo M, et al., Li Guohong*, and Li Pilong*. Rett syndrome mutations compromise MeCP2-mediated liquid-liquid phase separation of chromatin. Cell Research 2020, 30:393.

8. Wang L^#, Gao Y^#, Zheng X^#, et al., Li Haitao*, Li Pilong*. (2019) Histone modifications regulate chromatin compartmentalization by contributing to a phase separation mechanism. Molecular Cell, 76(4):646.

9. Fang Xiaofeng^#, Wang Liang^#, Ishikawa Ryo^#, et al., Li Pilong*, Dean Caroline*. (2019) Arabidopsis FLL2 promotes liquid-liquid phase separation of polyadenylation complexes. Nature, 569(7755):265.

10. Pilong Li^#, Sudeep Banjade^#, Hui-Chun Cheng^#, et al., B. TracyNixon, Michael K. Rosen. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483(7389):336.